polycarbonate membrane (Neuro Probe)
86
Structured Review
Neuro Probe
polycarbonate membrane
Polycarbonate Membrane, supplied by Neuro Probe, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polycarbonate+membrane/membrane+polycarbonate/10__3390_slash_chemistry8060080-104-19-28
Average 86 stars, based on 1 article reviews
Polycarbonate Membrane, supplied by Neuro Probe, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polycarbonate+membrane/membrane+polycarbonate/10__3390_slash_chemistry8060080-104-19-28
Average 86 stars, based on 1 article reviews
polycarbonate membrane - by Bioz Stars,
2026-11
86/100 stars
Images
Related Articles
Migration:Article Title: Proangiogenic Properties of Extracellular Vesicles Secreted by Endothelial Cells Reversibly Primed for Anoikis: A Possible Autocrine Mechanism Induced by Astrocytoma Extracellular Matrix. Article Snippet: CD63 and Annexin-2 exosomal markers were separated on 12% polyacrylamide resolving gels and detected with appropriate antibodies (anti-CD63, ref. #10628D, Thermo Scientific; anti-annexin-II, ref. # sc-9061, Santa Cruz Biotech, Dallas, TX, USA). .. For cell migration (chemotaxis) analysis in vitro, tubulogenesis-defective endothelial cells (TDECs) were seeded on the upper chamber at a density of 4 × 104 cells/well, in 48-well modified Boyden chambers containing a Article Title: A novel diselenide attenuates the carrageenan-induced inflammation by reducing neutrophil infiltration and the resulting tissue damage in mice. Article Snippet: selenium-containing compounds have emerged as promising treatment for redox-based and inflammatory diseases. this study aimed to investigate the in vitro and in vivo anti-inflammatory activity of a novel diselenide named as dibenzyl[diselanediyibis(propane-3-1diyl)] dicarbamate (DD).. DD reacted with hOcl (k = 9.2 x 107 M−1s−1), like glutathione (k = 1.2 x 108 M−1s−1), yielding seleninic and selenonic acid derivatives, and it also decreased hOcl formation by activated human neutrophils (ic50=4.6 μM) and purified myeloperoxidase (MPO) (ic50=3.8 μM). however, tyrosine, MPO-i and MPO-ii substrates, did not restore hOcl formation in presence of DD.. DD inhibited the oxidative burst in dhl-60 cells with no toxicity up to 25 μM for 48h. Article Title: Proangiogenic Properties of Extracellular Vesicles Secreted by Endothelial Cells Reversibly Primed for Anoikis: A Possible Autocrine Mechanism Induced by Astrocytoma Extracellular Matrix Article Snippet: CD63 and Annexin-2 exosomal markers were separated on 12% polyacrylamide resolving gels and detected with appropriate antibodies (anti-CD63, ref. #10628D, Thermo Scientific; anti-annexin-II, ref. # sc-9061, Santa Cruz Biotech, Dallas, TX, USA). .. For cell migration (chemotaxis) analysis in vitro, tubulogenesis-defective endothelial cells (TDECs) were seeded on the upper chamber at a density of 4 × 10 4 cells/well, in 48-well modified Boyden chambers containing a Chemotaxis Assay:Article Title: Proangiogenic Properties of Extracellular Vesicles Secreted by Endothelial Cells Reversibly Primed for Anoikis: A Possible Autocrine Mechanism Induced by Astrocytoma Extracellular Matrix. Article Snippet: CD63 and Annexin-2 exosomal markers were separated on 12% polyacrylamide resolving gels and detected with appropriate antibodies (anti-CD63, ref. #10628D, Thermo Scientific; anti-annexin-II, ref. # sc-9061, Santa Cruz Biotech, Dallas, TX, USA). .. For cell migration (chemotaxis) analysis in vitro, tubulogenesis-defective endothelial cells (TDECs) were seeded on the upper chamber at a density of 4 × 104 cells/well, in 48-well modified Boyden chambers containing a Article Title: A novel diselenide attenuates the carrageenan-induced inflammation by reducing neutrophil infiltration and the resulting tissue damage in mice. Article Snippet: selenium-containing compounds have emerged as promising treatment for redox-based and inflammatory diseases. this study aimed to investigate the in vitro and in vivo anti-inflammatory activity of a novel diselenide named as dibenzyl[diselanediyibis(propane-3-1diyl)] dicarbamate (DD).. DD reacted with hOcl (k = 9.2 x 107 M−1s−1), like glutathione (k = 1.2 x 108 M−1s−1), yielding seleninic and selenonic acid derivatives, and it also decreased hOcl formation by activated human neutrophils (ic50=4.6 μM) and purified myeloperoxidase (MPO) (ic50=3.8 μM). however, tyrosine, MPO-i and MPO-ii substrates, did not restore hOcl formation in presence of DD.. DD inhibited the oxidative burst in dhl-60 cells with no toxicity up to 25 μM for 48h. Article Title: Proangiogenic Properties of Extracellular Vesicles Secreted by Endothelial Cells Reversibly Primed for Anoikis: A Possible Autocrine Mechanism Induced by Astrocytoma Extracellular Matrix Article Snippet: CD63 and Annexin-2 exosomal markers were separated on 12% polyacrylamide resolving gels and detected with appropriate antibodies (anti-CD63, ref. #10628D, Thermo Scientific; anti-annexin-II, ref. # sc-9061, Santa Cruz Biotech, Dallas, TX, USA). .. For cell migration (chemotaxis) analysis in vitro, tubulogenesis-defective endothelial cells (TDECs) were seeded on the upper chamber at a density of 4 × 10 4 cells/well, in 48-well modified Boyden chambers containing a In Vitro:Article Title: Proangiogenic Properties of Extracellular Vesicles Secreted by Endothelial Cells Reversibly Primed for Anoikis: A Possible Autocrine Mechanism Induced by Astrocytoma Extracellular Matrix. Article Snippet: CD63 and Annexin-2 exosomal markers were separated on 12% polyacrylamide resolving gels and detected with appropriate antibodies (anti-CD63, ref. #10628D, Thermo Scientific; anti-annexin-II, ref. # sc-9061, Santa Cruz Biotech, Dallas, TX, USA). .. For cell migration (chemotaxis) analysis in vitro, tubulogenesis-defective endothelial cells (TDECs) were seeded on the upper chamber at a density of 4 × 104 cells/well, in 48-well modified Boyden chambers containing a Article Title: A novel diselenide attenuates the carrageenan-induced inflammation by reducing neutrophil infiltration and the resulting tissue damage in mice. Article Snippet: selenium-containing compounds have emerged as promising treatment for redox-based and inflammatory diseases. this study aimed to investigate the in vitro and in vivo anti-inflammatory activity of a novel diselenide named as dibenzyl[diselanediyibis(propane-3-1diyl)] dicarbamate (DD).. DD reacted with hOcl (k = 9.2 x 107 M−1s−1), like glutathione (k = 1.2 x 108 M−1s−1), yielding seleninic and selenonic acid derivatives, and it also decreased hOcl formation by activated human neutrophils (ic50=4.6 μM) and purified myeloperoxidase (MPO) (ic50=3.8 μM). however, tyrosine, MPO-i and MPO-ii substrates, did not restore hOcl formation in presence of DD.. DD inhibited the oxidative burst in dhl-60 cells with no toxicity up to 25 μM for 48h. Article Title: Proangiogenic Properties of Extracellular Vesicles Secreted by Endothelial Cells Reversibly Primed for Anoikis: A Possible Autocrine Mechanism Induced by Astrocytoma Extracellular Matrix Article Snippet: CD63 and Annexin-2 exosomal markers were separated on 12% polyacrylamide resolving gels and detected with appropriate antibodies (anti-CD63, ref. #10628D, Thermo Scientific; anti-annexin-II, ref. # sc-9061, Santa Cruz Biotech, Dallas, TX, USA). .. For cell migration (chemotaxis) analysis in vitro, tubulogenesis-defective endothelial cells (TDECs) were seeded on the upper chamber at a density of 4 × 10 4 cells/well, in 48-well modified Boyden chambers containing a Modification:Article Title: Proangiogenic Properties of Extracellular Vesicles Secreted by Endothelial Cells Reversibly Primed for Anoikis: A Possible Autocrine Mechanism Induced by Astrocytoma Extracellular Matrix. Article Snippet: CD63 and Annexin-2 exosomal markers were separated on 12% polyacrylamide resolving gels and detected with appropriate antibodies (anti-CD63, ref. #10628D, Thermo Scientific; anti-annexin-II, ref. # sc-9061, Santa Cruz Biotech, Dallas, TX, USA). .. For cell migration (chemotaxis) analysis in vitro, tubulogenesis-defective endothelial cells (TDECs) were seeded on the upper chamber at a density of 4 × 104 cells/well, in 48-well modified Boyden chambers containing a Article Title: A novel diselenide attenuates the carrageenan-induced inflammation by reducing neutrophil infiltration and the resulting tissue damage in mice. Article Snippet: selenium-containing compounds have emerged as promising treatment for redox-based and inflammatory diseases. this study aimed to investigate the in vitro and in vivo anti-inflammatory activity of a novel diselenide named as dibenzyl[diselanediyibis(propane-3-1diyl)] dicarbamate (DD).. DD reacted with hOcl (k = 9.2 x 107 M−1s−1), like glutathione (k = 1.2 x 108 M−1s−1), yielding seleninic and selenonic acid derivatives, and it also decreased hOcl formation by activated human neutrophils (ic50=4.6 μM) and purified myeloperoxidase (MPO) (ic50=3.8 μM). however, tyrosine, MPO-i and MPO-ii substrates, did not restore hOcl formation in presence of DD.. DD inhibited the oxidative burst in dhl-60 cells with no toxicity up to 25 μM for 48h. Article Title: Proangiogenic Properties of Extracellular Vesicles Secreted by Endothelial Cells Reversibly Primed for Anoikis: A Possible Autocrine Mechanism Induced by Astrocytoma Extracellular Matrix Article Snippet: CD63 and Annexin-2 exosomal markers were separated on 12% polyacrylamide resolving gels and detected with appropriate antibodies (anti-CD63, ref. #10628D, Thermo Scientific; anti-annexin-II, ref. # sc-9061, Santa Cruz Biotech, Dallas, TX, USA). .. For cell migration (chemotaxis) analysis in vitro, tubulogenesis-defective endothelial cells (TDECs) were seeded on the upper chamber at a density of 4 × 10 4 cells/well, in 48-well modified Boyden chambers containing a Membrane:Article Title: Proangiogenic Properties of Extracellular Vesicles Secreted by Endothelial Cells Reversibly Primed for Anoikis: A Possible Autocrine Mechanism Induced by Astrocytoma Extracellular Matrix. Article Snippet: CD63 and Annexin-2 exosomal markers were separated on 12% polyacrylamide resolving gels and detected with appropriate antibodies (anti-CD63, ref. #10628D, Thermo Scientific; anti-annexin-II, ref. # sc-9061, Santa Cruz Biotech, Dallas, TX, USA). .. For cell migration (chemotaxis) analysis in vitro, tubulogenesis-defective endothelial cells (TDECs) were seeded on the upper chamber at a density of 4 × 104 cells/well, in 48-well modified Boyden chambers containing a Article Title: Glycosaminoglycan binding to soluble CX3CL1 impacts monocyte migration in vitro . Article Snippet: The THP-1 monocytic cell line was purchased from the European collection of cell cultures and grown in RPMI 1640 medium (Sigma) containing 10% fetal calf serum, 2 mM glutamine, 100 units/ml penicillin, and 100 units/ml streptomycin at 37 °C and 5% CO2. .. To investigate the chemotactic response of monocytes – bloodderived monocytes or THP1monocytic cell line – to cdCX3CL1 and mutants thereof, a 48-well Boyden chamber (Neuroprobe, Gaithersburg, MD, USA) and a Article Title: A novel diselenide attenuates the carrageenan-induced inflammation by reducing neutrophil infiltration and the resulting tissue damage in mice. Article Snippet: selenium-containing compounds have emerged as promising treatment for redox-based and inflammatory diseases. this study aimed to investigate the in vitro and in vivo anti-inflammatory activity of a novel diselenide named as dibenzyl[diselanediyibis(propane-3-1diyl)] dicarbamate (DD).. DD reacted with hOcl (k = 9.2 x 107 M−1s−1), like glutathione (k = 1.2 x 108 M−1s−1), yielding seleninic and selenonic acid derivatives, and it also decreased hOcl formation by activated human neutrophils (ic50=4.6 μM) and purified myeloperoxidase (MPO) (ic50=3.8 μM). however, tyrosine, MPO-i and MPO-ii substrates, did not restore hOcl formation in presence of DD.. DD inhibited the oxidative burst in dhl-60 cells with no toxicity up to 25 μM for 48h. Article Title: Proangiogenic Properties of Extracellular Vesicles Secreted by Endothelial Cells Reversibly Primed for Anoikis: A Possible Autocrine Mechanism Induced by Astrocytoma Extracellular Matrix Article Snippet: CD63 and Annexin-2 exosomal markers were separated on 12% polyacrylamide resolving gels and detected with appropriate antibodies (anti-CD63, ref. #10628D, Thermo Scientific; anti-annexin-II, ref. # sc-9061, Santa Cruz Biotech, Dallas, TX, USA). .. For cell migration (chemotaxis) analysis in vitro, tubulogenesis-defective endothelial cells (TDECs) were seeded on the upper chamber at a density of 4 × 10 4 cells/well, in 48-well modified Boyden chambers containing a Article Title: Low-Molecular-Weight Versus Protein Inhibitors for the CXCL8/Glycosaminoglycan Interaction: Biophysical Characterization and Cellular Activity Article Snippet: .. To investigate the chemotactic response of neutrophils to CXCL8, a 48-well Boyden chamber (Neuroprobe, Gaithersburg, MD, USA) and a Article Title: A Novel Image‐Based Approach for Analyzing Neutrophil Chemotaxis Using a Boyden Chamber Assay Article Snippet: .. Ketamine Xylazine Acepromazine Phosphate‐buffered saline (PBS) without Ca 2+ and Mg 2+ (Sigma, cat. no. P3813) C57BL6/J mice (Jackson Laboratory, strain 000664) 70% (v/v) ethanol Neutrophil recommended medium (NRM, see recipe) MojoSort Mouse Neutrophil Isolation Kit (BioLegend cat. no. 480058) including biotin antibody cocktail and streptavidin nanobeads Mojo buffer (see recipe) Trypan blue solution (Sigma, cat. no. T8154) N ‐Formylmethionyl‐leucyl‐phenylalanine (fMLP, Sigma, cat. no. F3506) Hemacolor stain set (EMD Millipore Sigma) Mounting medium 23‐G needles 3‐ml syringes Sterile surgical scissors, forceps, and scalpel Sterile Petri dishes 40‐μm cell strainers (Falcon, cat. no. 352340) 50‐ml Falcon tubes Hemocytometer Cell separation magnet (Stemcell Technologies) Boyden chamber (Neuro Probe, cat. no. AP48) Article Title: GRK6 palmitoylation dictates triple-negative breast cancer metastasis via recruiting the β-Arrestin 2/MAPKs/NF-κB signaling axis Article Snippet: Cellular migration assay was performed in the trans-well culture using Boyden chamber system (Neuro Probe, Inc., Gaithersburg, MD, USA). .. Briefly, the lower chamber was fulfilled the conditioned media prior to covering with a Article Title: Anthraflavic Acid Exerts Antimetastatic Effects on Human Non-Small-cell Lung Cancer Cells Via Activation of Aryl Hydrocarbon Receptor and Regulation of the JNK, p38, and c-myc Signaling Pathways. Article Snippet: Anthraquinone is a polycyclic aromatic hydrocarbon compound found in plants and animals [1].. Derivatives of anthraquinon exert various pharmaceutical activities such as anti-microbial, anti-inflammatory, anticancer, and antioxidant effects [2].. Anthraflavic acid (AFA), an anthraquinone derivative, can be extracted from plants including Rubia tinctorum and Senna obtusifolia (Fig. 1A) [3, 4]. Pore Size:Article Title: Proangiogenic Properties of Extracellular Vesicles Secreted by Endothelial Cells Reversibly Primed for Anoikis: A Possible Autocrine Mechanism Induced by Astrocytoma Extracellular Matrix. Article Snippet: CD63 and Annexin-2 exosomal markers were separated on 12% polyacrylamide resolving gels and detected with appropriate antibodies (anti-CD63, ref. #10628D, Thermo Scientific; anti-annexin-II, ref. # sc-9061, Santa Cruz Biotech, Dallas, TX, USA). .. For cell migration (chemotaxis) analysis in vitro, tubulogenesis-defective endothelial cells (TDECs) were seeded on the upper chamber at a density of 4 × 104 cells/well, in 48-well modified Boyden chambers containing a Article Title: Glycosaminoglycan binding to soluble CX3CL1 impacts monocyte migration in vitro . Article Snippet: The THP-1 monocytic cell line was purchased from the European collection of cell cultures and grown in RPMI 1640 medium (Sigma) containing 10% fetal calf serum, 2 mM glutamine, 100 units/ml penicillin, and 100 units/ml streptomycin at 37 °C and 5% CO2. .. To investigate the chemotactic response of monocytes – bloodderived monocytes or THP1monocytic cell line – to cdCX3CL1 and mutants thereof, a 48-well Boyden chamber (Neuroprobe, Gaithersburg, MD, USA) and a Article Title: Proangiogenic Properties of Extracellular Vesicles Secreted by Endothelial Cells Reversibly Primed for Anoikis: A Possible Autocrine Mechanism Induced by Astrocytoma Extracellular Matrix Article Snippet: CD63 and Annexin-2 exosomal markers were separated on 12% polyacrylamide resolving gels and detected with appropriate antibodies (anti-CD63, ref. #10628D, Thermo Scientific; anti-annexin-II, ref. # sc-9061, Santa Cruz Biotech, Dallas, TX, USA). .. For cell migration (chemotaxis) analysis in vitro, tubulogenesis-defective endothelial cells (TDECs) were seeded on the upper chamber at a density of 4 × 10 4 cells/well, in 48-well modified Boyden chambers containing a Article Title: Low-Molecular-Weight Versus Protein Inhibitors for the CXCL8/Glycosaminoglycan Interaction: Biophysical Characterization and Cellular Activity Article Snippet: .. To investigate the chemotactic response of neutrophils to CXCL8, a 48-well Boyden chamber (Neuroprobe, Gaithersburg, MD, USA) and a Article Title: GRK6 palmitoylation dictates triple-negative breast cancer metastasis via recruiting the β-Arrestin 2/MAPKs/NF-κB signaling axis Article Snippet: Cellular migration assay was performed in the trans-well culture using Boyden chamber system (Neuro Probe, Inc., Gaithersburg, MD, USA). .. Briefly, the lower chamber was fulfilled the conditioned media prior to covering with a Positive Control:Article Title: Proangiogenic Properties of Extracellular Vesicles Secreted by Endothelial Cells Reversibly Primed for Anoikis: A Possible Autocrine Mechanism Induced by Astrocytoma Extracellular Matrix. Article Snippet: CD63 and Annexin-2 exosomal markers were separated on 12% polyacrylamide resolving gels and detected with appropriate antibodies (anti-CD63, ref. #10628D, Thermo Scientific; anti-annexin-II, ref. # sc-9061, Santa Cruz Biotech, Dallas, TX, USA). .. For cell migration (chemotaxis) analysis in vitro, tubulogenesis-defective endothelial cells (TDECs) were seeded on the upper chamber at a density of 4 × 104 cells/well, in 48-well modified Boyden chambers containing a Article Title: A novel diselenide attenuates the carrageenan-induced inflammation by reducing neutrophil infiltration and the resulting tissue damage in mice. Article Snippet: selenium-containing compounds have emerged as promising treatment for redox-based and inflammatory diseases. this study aimed to investigate the in vitro and in vivo anti-inflammatory activity of a novel diselenide named as dibenzyl[diselanediyibis(propane-3-1diyl)] dicarbamate (DD).. DD reacted with hOcl (k = 9.2 x 107 M−1s−1), like glutathione (k = 1.2 x 108 M−1s−1), yielding seleninic and selenonic acid derivatives, and it also decreased hOcl formation by activated human neutrophils (ic50=4.6 μM) and purified myeloperoxidase (MPO) (ic50=3.8 μM). however, tyrosine, MPO-i and MPO-ii substrates, did not restore hOcl formation in presence of DD.. DD inhibited the oxidative burst in dhl-60 cells with no toxicity up to 25 μM for 48h. Article Title: Proangiogenic Properties of Extracellular Vesicles Secreted by Endothelial Cells Reversibly Primed for Anoikis: A Possible Autocrine Mechanism Induced by Astrocytoma Extracellular Matrix Article Snippet: CD63 and Annexin-2 exosomal markers were separated on 12% polyacrylamide resolving gels and detected with appropriate antibodies (anti-CD63, ref. #10628D, Thermo Scientific; anti-annexin-II, ref. # sc-9061, Santa Cruz Biotech, Dallas, TX, USA). .. For cell migration (chemotaxis) analysis in vitro, tubulogenesis-defective endothelial cells (TDECs) were seeded on the upper chamber at a density of 4 × 10 4 cells/well, in 48-well modified Boyden chambers containing a Negative Control:Article Title: Proangiogenic Properties of Extracellular Vesicles Secreted by Endothelial Cells Reversibly Primed for Anoikis: A Possible Autocrine Mechanism Induced by Astrocytoma Extracellular Matrix. Article Snippet: CD63 and Annexin-2 exosomal markers were separated on 12% polyacrylamide resolving gels and detected with appropriate antibodies (anti-CD63, ref. #10628D, Thermo Scientific; anti-annexin-II, ref. # sc-9061, Santa Cruz Biotech, Dallas, TX, USA). .. For cell migration (chemotaxis) analysis in vitro, tubulogenesis-defective endothelial cells (TDECs) were seeded on the upper chamber at a density of 4 × 104 cells/well, in 48-well modified Boyden chambers containing a Article Title: A novel diselenide attenuates the carrageenan-induced inflammation by reducing neutrophil infiltration and the resulting tissue damage in mice. Article Snippet: selenium-containing compounds have emerged as promising treatment for redox-based and inflammatory diseases. this study aimed to investigate the in vitro and in vivo anti-inflammatory activity of a novel diselenide named as dibenzyl[diselanediyibis(propane-3-1diyl)] dicarbamate (DD).. DD reacted with hOcl (k = 9.2 x 107 M−1s−1), like glutathione (k = 1.2 x 108 M−1s−1), yielding seleninic and selenonic acid derivatives, and it also decreased hOcl formation by activated human neutrophils (ic50=4.6 μM) and purified myeloperoxidase (MPO) (ic50=3.8 μM). however, tyrosine, MPO-i and MPO-ii substrates, did not restore hOcl formation in presence of DD.. DD inhibited the oxidative burst in dhl-60 cells with no toxicity up to 25 μM for 48h. Article Title: Proangiogenic Properties of Extracellular Vesicles Secreted by Endothelial Cells Reversibly Primed for Anoikis: A Possible Autocrine Mechanism Induced by Astrocytoma Extracellular Matrix Article Snippet: CD63 and Annexin-2 exosomal markers were separated on 12% polyacrylamide resolving gels and detected with appropriate antibodies (anti-CD63, ref. #10628D, Thermo Scientific; anti-annexin-II, ref. # sc-9061, Santa Cruz Biotech, Dallas, TX, USA). .. For cell migration (chemotaxis) analysis in vitro, tubulogenesis-defective endothelial cells (TDECs) were seeded on the upper chamber at a density of 4 × 10 4 cells/well, in 48-well modified Boyden chambers containing a Isolation:Article Title: Proangiogenic Properties of Extracellular Vesicles Secreted by Endothelial Cells Reversibly Primed for Anoikis: A Possible Autocrine Mechanism Induced by Astrocytoma Extracellular Matrix. Article Snippet: CD63 and Annexin-2 exosomal markers were separated on 12% polyacrylamide resolving gels and detected with appropriate antibodies (anti-CD63, ref. #10628D, Thermo Scientific; anti-annexin-II, ref. # sc-9061, Santa Cruz Biotech, Dallas, TX, USA). .. For cell migration (chemotaxis) analysis in vitro, tubulogenesis-defective endothelial cells (TDECs) were seeded on the upper chamber at a density of 4 × 104 cells/well, in 48-well modified Boyden chambers containing a Article Title: Proangiogenic Properties of Extracellular Vesicles Secreted by Endothelial Cells Reversibly Primed for Anoikis: A Possible Autocrine Mechanism Induced by Astrocytoma Extracellular Matrix Article Snippet: CD63 and Annexin-2 exosomal markers were separated on 12% polyacrylamide resolving gels and detected with appropriate antibodies (anti-CD63, ref. #10628D, Thermo Scientific; anti-annexin-II, ref. # sc-9061, Santa Cruz Biotech, Dallas, TX, USA). .. For cell migration (chemotaxis) analysis in vitro, tubulogenesis-defective endothelial cells (TDECs) were seeded on the upper chamber at a density of 4 × 10 4 cells/well, in 48-well modified Boyden chambers containing a Article Title: A Novel Image‐Based Approach for Analyzing Neutrophil Chemotaxis Using a Boyden Chamber Assay Article Snippet: .. Ketamine Xylazine Acepromazine Phosphate‐buffered saline (PBS) without Ca 2+ and Mg 2+ (Sigma, cat. no. P3813) C57BL6/J mice (Jackson Laboratory, strain 000664) 70% (v/v) ethanol Neutrophil recommended medium (NRM, see recipe) MojoSort Mouse Neutrophil Isolation Kit (BioLegend cat. no. 480058) including biotin antibody cocktail and streptavidin nanobeads Mojo buffer (see recipe) Trypan blue solution (Sigma, cat. no. T8154) N ‐Formylmethionyl‐leucyl‐phenylalanine (fMLP, Sigma, cat. no. F3506) Hemacolor stain set (EMD Millipore Sigma) Mounting medium 23‐G needles 3‐ml syringes Sterile surgical scissors, forceps, and scalpel Sterile Petri dishes 40‐μm cell strainers (Falcon, cat. no. 352340) 50‐ml Falcon tubes Hemocytometer Cell separation magnet (Stemcell Technologies) Boyden chamber (Neuro Probe, cat. no. AP48) Suspension:Article Title: A novel diselenide attenuates the carrageenan-induced inflammation by reducing neutrophil infiltration and the resulting tissue damage in mice. Article Snippet: selenium-containing compounds have emerged as promising treatment for redox-based and inflammatory diseases. this study aimed to investigate the in vitro and in vivo anti-inflammatory activity of a novel diselenide named as dibenzyl[diselanediyibis(propane-3-1diyl)] dicarbamate (DD).. DD reacted with hOcl (k = 9.2 x 107 M−1s−1), like glutathione (k = 1.2 x 108 M−1s−1), yielding seleninic and selenonic acid derivatives, and it also decreased hOcl formation by activated human neutrophils (ic50=4.6 μM) and purified myeloperoxidase (MPO) (ic50=3.8 μM). however, tyrosine, MPO-i and MPO-ii substrates, did not restore hOcl formation in presence of DD.. DD inhibited the oxidative burst in dhl-60 cells with no toxicity up to 25 μM for 48h. Incubation:Article Title: A novel diselenide attenuates the carrageenan-induced inflammation by reducing neutrophil infiltration and the resulting tissue damage in mice. Article Snippet: selenium-containing compounds have emerged as promising treatment for redox-based and inflammatory diseases. this study aimed to investigate the in vitro and in vivo anti-inflammatory activity of a novel diselenide named as dibenzyl[diselanediyibis(propane-3-1diyl)] dicarbamate (DD).. DD reacted with hOcl (k = 9.2 x 107 M−1s−1), like glutathione (k = 1.2 x 108 M−1s−1), yielding seleninic and selenonic acid derivatives, and it also decreased hOcl formation by activated human neutrophils (ic50=4.6 μM) and purified myeloperoxidase (MPO) (ic50=3.8 μM). however, tyrosine, MPO-i and MPO-ii substrates, did not restore hOcl formation in presence of DD.. DD inhibited the oxidative burst in dhl-60 cells with no toxicity up to 25 μM for 48h. Staining:Article Title: A novel diselenide attenuates the carrageenan-induced inflammation by reducing neutrophil infiltration and the resulting tissue damage in mice. Article Snippet: selenium-containing compounds have emerged as promising treatment for redox-based and inflammatory diseases. this study aimed to investigate the in vitro and in vivo anti-inflammatory activity of a novel diselenide named as dibenzyl[diselanediyibis(propane-3-1diyl)] dicarbamate (DD).. DD reacted with hOcl (k = 9.2 x 107 M−1s−1), like glutathione (k = 1.2 x 108 M−1s−1), yielding seleninic and selenonic acid derivatives, and it also decreased hOcl formation by activated human neutrophils (ic50=4.6 μM) and purified myeloperoxidase (MPO) (ic50=3.8 μM). however, tyrosine, MPO-i and MPO-ii substrates, did not restore hOcl formation in presence of DD.. DD inhibited the oxidative burst in dhl-60 cells with no toxicity up to 25 μM for 48h. Article Title: A Novel Image‐Based Approach for Analyzing Neutrophil Chemotaxis Using a Boyden Chamber Assay Article Snippet: .. Ketamine Xylazine Acepromazine Phosphate‐buffered saline (PBS) without Ca 2+ and Mg 2+ (Sigma, cat. no. P3813) C57BL6/J mice (Jackson Laboratory, strain 000664) 70% (v/v) ethanol Neutrophil recommended medium (NRM, see recipe) MojoSort Mouse Neutrophil Isolation Kit (BioLegend cat. no. 480058) including biotin antibody cocktail and streptavidin nanobeads Mojo buffer (see recipe) Trypan blue solution (Sigma, cat. no. T8154) N ‐Formylmethionyl‐leucyl‐phenylalanine (fMLP, Sigma, cat. no. F3506) Hemacolor stain set (EMD Millipore Sigma) Mounting medium 23‐G needles 3‐ml syringes Sterile surgical scissors, forceps, and scalpel Sterile Petri dishes 40‐μm cell strainers (Falcon, cat. no. 352340) 50‐ml Falcon tubes Hemocytometer Cell separation magnet (Stemcell Technologies) Boyden chamber (Neuro Probe, cat. no. AP48) Microscopy:Article Title: A novel diselenide attenuates the carrageenan-induced inflammation by reducing neutrophil infiltration and the resulting tissue damage in mice. Article Snippet: selenium-containing compounds have emerged as promising treatment for redox-based and inflammatory diseases. this study aimed to investigate the in vitro and in vivo anti-inflammatory activity of a novel diselenide named as dibenzyl[diselanediyibis(propane-3-1diyl)] dicarbamate (DD).. DD reacted with hOcl (k = 9.2 x 107 M−1s−1), like glutathione (k = 1.2 x 108 M−1s−1), yielding seleninic and selenonic acid derivatives, and it also decreased hOcl formation by activated human neutrophils (ic50=4.6 μM) and purified myeloperoxidase (MPO) (ic50=3.8 μM). however, tyrosine, MPO-i and MPO-ii substrates, did not restore hOcl formation in presence of DD.. DD inhibited the oxidative burst in dhl-60 cells with no toxicity up to 25 μM for 48h. Article Title: A Novel Image‐Based Approach for Analyzing Neutrophil Chemotaxis Using a Boyden Chamber Assay Article Snippet: .. Ketamine Xylazine Acepromazine Phosphate‐buffered saline (PBS) without Ca 2+ and Mg 2+ (Sigma, cat. no. P3813) C57BL6/J mice (Jackson Laboratory, strain 000664) 70% (v/v) ethanol Neutrophil recommended medium (NRM, see recipe) MojoSort Mouse Neutrophil Isolation Kit (BioLegend cat. no. 480058) including biotin antibody cocktail and streptavidin nanobeads Mojo buffer (see recipe) Trypan blue solution (Sigma, cat. no. T8154) N ‐Formylmethionyl‐leucyl‐phenylalanine (fMLP, Sigma, cat. no. F3506) Hemacolor stain set (EMD Millipore Sigma) Mounting medium 23‐G needles 3‐ml syringes Sterile surgical scissors, forceps, and scalpel Sterile Petri dishes 40‐μm cell strainers (Falcon, cat. no. 352340) 50‐ml Falcon tubes Hemocytometer Cell separation magnet (Stemcell Technologies) Boyden chamber (Neuro Probe, cat. no. AP48) Saline:Article Title: A Novel Image‐Based Approach for Analyzing Neutrophil Chemotaxis Using a Boyden Chamber Assay Article Snippet: .. Ketamine Xylazine Acepromazine Phosphate‐buffered saline (PBS) without Ca 2+ and Mg 2+ (Sigma, cat. no. P3813) C57BL6/J mice (Jackson Laboratory, strain 000664) 70% (v/v) ethanol Neutrophil recommended medium (NRM, see recipe) MojoSort Mouse Neutrophil Isolation Kit (BioLegend cat. no. 480058) including biotin antibody cocktail and streptavidin nanobeads Mojo buffer (see recipe) Trypan blue solution (Sigma, cat. no. T8154) N ‐Formylmethionyl‐leucyl‐phenylalanine (fMLP, Sigma, cat. no. F3506) Hemacolor stain set (EMD Millipore Sigma) Mounting medium 23‐G needles 3‐ml syringes Sterile surgical scissors, forceps, and scalpel Sterile Petri dishes 40‐μm cell strainers (Falcon, cat. no. 352340) 50‐ml Falcon tubes Hemocytometer Cell separation magnet (Stemcell Technologies) Boyden chamber (Neuro Probe, cat. no. AP48) Sterility:Article Title: A Novel Image‐Based Approach for Analyzing Neutrophil Chemotaxis Using a Boyden Chamber Assay Article Snippet: .. Ketamine Xylazine Acepromazine Phosphate‐buffered saline (PBS) without Ca 2+ and Mg 2+ (Sigma, cat. no. P3813) C57BL6/J mice (Jackson Laboratory, strain 000664) 70% (v/v) ethanol Neutrophil recommended medium (NRM, see recipe) MojoSort Mouse Neutrophil Isolation Kit (BioLegend cat. no. 480058) including biotin antibody cocktail and streptavidin nanobeads Mojo buffer (see recipe) Trypan blue solution (Sigma, cat. no. T8154) N ‐Formylmethionyl‐leucyl‐phenylalanine (fMLP, Sigma, cat. no. F3506) Hemacolor stain set (EMD Millipore Sigma) Mounting medium 23‐G needles 3‐ml syringes Sterile surgical scissors, forceps, and scalpel Sterile Petri dishes 40‐μm cell strainers (Falcon, cat. no. 352340) 50‐ml Falcon tubes Hemocytometer Cell separation magnet (Stemcell Technologies) Boyden chamber (Neuro Probe, cat. no. AP48) Software:Article Title: A Novel Image‐Based Approach for Analyzing Neutrophil Chemotaxis Using a Boyden Chamber Assay Article Snippet: .. Ketamine Xylazine Acepromazine Phosphate‐buffered saline (PBS) without Ca 2+ and Mg 2+ (Sigma, cat. no. P3813) C57BL6/J mice (Jackson Laboratory, strain 000664) 70% (v/v) ethanol Neutrophil recommended medium (NRM, see recipe) MojoSort Mouse Neutrophil Isolation Kit (BioLegend cat. no. 480058) including biotin antibody cocktail and streptavidin nanobeads Mojo buffer (see recipe) Trypan blue solution (Sigma, cat. no. T8154) N ‐Formylmethionyl‐leucyl‐phenylalanine (fMLP, Sigma, cat. no. F3506) Hemacolor stain set (EMD Millipore Sigma) Mounting medium 23‐G needles 3‐ml syringes Sterile surgical scissors, forceps, and scalpel Sterile Petri dishes 40‐μm cell strainers (Falcon, cat. no. 352340) 50‐ml Falcon tubes Hemocytometer Cell separation magnet (Stemcell Technologies) Boyden chamber (Neuro Probe, cat. no. AP48) Hood:Article Title: A Novel Image‐Based Approach for Analyzing Neutrophil Chemotaxis Using a Boyden Chamber Assay Article Snippet: .. Ketamine Xylazine Acepromazine Phosphate‐buffered saline (PBS) without Ca 2+ and Mg 2+ (Sigma, cat. no. P3813) C57BL6/J mice (Jackson Laboratory, strain 000664) 70% (v/v) ethanol Neutrophil recommended medium (NRM, see recipe) MojoSort Mouse Neutrophil Isolation Kit (BioLegend cat. no. 480058) including biotin antibody cocktail and streptavidin nanobeads Mojo buffer (see recipe) Trypan blue solution (Sigma, cat. no. T8154) N ‐Formylmethionyl‐leucyl‐phenylalanine (fMLP, Sigma, cat. no. F3506) Hemacolor stain set (EMD Millipore Sigma) Mounting medium 23‐G needles 3‐ml syringes Sterile surgical scissors, forceps, and scalpel Sterile Petri dishes 40‐μm cell strainers (Falcon, cat. no. 352340) 50‐ml Falcon tubes Hemocytometer Cell separation magnet (Stemcell Technologies) Boyden chamber (Neuro Probe, cat. no. AP48) |